High Throughput Screening Robotics Core

The core will allow for automated screening of cells cultured in 96 or 384 plate formants and can be applied to multiple investigator-initiated applications throughout the university community. The applications include:

1) small molecule drug screens applied to cells engineered by WU investigators

2) small molecule or peptide screens to identify enzyme inhibitors (directed against high priority proteases, kinases, phosphatases, etc.), or modulators of protein-protein interactions

3) genome-wide siRNA library screens against small molecules in mammalian cells targeting cell growth or cell death responses

Platforms incorporating biolumninescence or fluorescence HTS readouts are available, and an HTS core can be coupled with high content cell screens such as the automated, computerized Zeiss microscope offered by Cellomics. These systems enable a computer to be trained to recognize morphological changes at the cellular level, allowing automated screens of changes in cell biological properties.


		Click here to see robot in action (big file--high speed connection recommended)
		--to download from a Mac control click on link and choose "Download linked files as" --to download from a PC right click and choose "Save target as"
		The core will allow for automated screening of cells cultured in 96 or 384 plate formants and can be applied to multiple investigator-initiated applications throughout the university community. The applications include: 1) small molecule drug screens applied to cells engineered by WU investigators 2) small molecule or peptide screens to identify enzyme inhibitors (directed against high priority proteases, kinases, phosphatases, etc.), or modulators of protein-protein interactions 3) genome-wide siRNA library screens against small molecules in mammalian cells targeting cell growth or cell death responses Platforms incorporating biolumninescence or fluorescence HTS readouts are available, and an HTS core can be coupled with high content cell screens such as the automated, computerized Zeiss microscope offered by Cellomics. These systems enable a computer to be trained to recognize morphological changes at the cellular level, allowing automated screens of changes in cell biological properties.