PNACL Order Forms:

Protein Sequencing

DNA Sequencing (2 pages)

 

The above order forms are in .pdf format. You'll need Adobe Reader 5.0 or later to view, fill out and print the documents.

Adobe Reader is free at the Adobe website. You may download Adobe Reader at the following address:

http://www.adobe.com/prodindex/acrobat/readstep.html

 

PNACL DNA Sequencing Links:

To download Edit View 1.0.1, ABI's sequence viewer freeware:

http://www.appliedbiosystems.com/support/download/SeqAnal/installs/mac/EditView_1.0.1.sea.hqx

To download protocols for ABI Prism BigDye Terminator sequencing kits in .pdf format:

http://docs.appliedbiosystems.com/search.taf?_UserReference=1CEA59CCC78720C93BF142D2

To Open and Edit your ABI Sequencing Data Files

For Macs you need software called "Editview." It's freely available from the Applied Biosystems website and allows you to open the electropherogram files, manually edit them and print them. It can be found on the DNA Sequencing and Fragment Analysis Software page of the Applied Biosystems website:

http://www.appliedbiosystems.com/support/software/dnaseq/installs.cfm

Go to this URL and look for "EditView - an annotated DNA sequence viewer".

For PCs you can use the freeware version of Traceviewer which is available from CodonCode.com at this URL:

http://www.codoncode.com/TraceViewer/

Another freeware for PCs is called BioEdit. This one will allow editing and printing as well as sequence alignment. It is a nice piece of freeware but is not as user friendly as some of the others.

http://www.mbio.ncsu.edu/BioEdit/bioedit.html

Another PC program is called Chromas. You can download the freeware version and use it for a demo several times. After that, they request you pay a nominal user's fee (I think it's $50) to keep using it. It has more features than the ABI View and does allow printing. The Chromas home page is:

http://www.technelysium.com.au/chromas.html

The Staden Package is a package of sequence alignment, etc. software which is freely available to academic labs. It is based on the Unix operating system and can be used on Unix and PCs. You can download it from:

http://www.mrc-lmb.cam.ac.uk/pubseq/staden_home.html

There are several sequence alignment software packages available that will import ABI sequencing files (text and trace).

DNA Star, and Sequencher (GeneCodes) are available for both Macs and PCs. These are two pleasant-to-use, reasonably priced software packages ($2000-$3000, single license). Vector NTI (Informax) is a hot new, overly powerful software suite that does so much it can be difficult to do anything simple. It's also more expensive.

Finch TV (MAC and PC users)

To vew and print out electropherogram.

http://www.geospiza.com/finchtv

 

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