Protocols

ChIP Protocols

• Kroll Lab ChIP Protocols (Word)

ES and P19 Cell Culture

ES and P19 Culture Combined

• BD RevTet System (PDF)
• ES and P19 combined 12-2007 (Word)

Transfection Reagents

• FuGENE6 (PDF)
• Fusion Brochure (Pierce) (PDF)
• Lipofectamine (Invitrogen) (PDF)
• Polyfect (Qiagen) (PDF)

Molecular Biology

Cloning

• Combined Molecular Biology - Short (Word)
• Gel Extraction (Qiagen) (PDF)
• PCR Purification (Qiagen, Invitrogen) (PDF)
• T4 DNS Ligase-NEB (PDF)
• TOPO TA Cloning (Invitrogen) (PDF)

Genomic DNA Isolation

• DNA Isolation-Mouse Tails (PDF)
• Endofree Plasmid Maxi (PDF)
• ES Cell Genomic DNA (PDF)
• Genomic DNA Isolation-Blood (PDF)
• Genomic-tip Protocol (PDF)

RNA and qRTPCR Protocols

• Capped RNA (Ambion) (PDF)
• cDNA Prep Using RT (Qiagen) (PDF)
• Combined RNA Isolation and qRTPCR Short (Word)
• Real-time PCR Overview (Word)
• SuperScript II (RT) (Gibco) (PDF)
• Total RNA Prep (Qiagen) (PDF)
• Trizol (Invitrogen) (PDF)

Protein Methods

• 2007 Combined Protein Methods

Protein Assay Guides

• Beta-gal Assay (Promega) (PDF)
• Beta-gal Assay (PDF)
• Bradford Assay-Biorad (PDF)
• Dual Luciferase-Protocol (Word)
• Dual Luciferase (Promega) (PDF)
• Dual Luciferase (PDF)
• ECL Plus (PDF)
• GST Handbook (Amersham) (PDF)
• GST Purification Module (PDF)
• GST Bind Kit (Novagen) (PDF)
• Luciferase Assay (Promega) (PDF)

Xenopus

Xenopus IVF and Embryo Microinjection

• Xenopus Laevis IVF Injection Quick References 12-2007 (Word)
• Xenopus Tripicalis Quick Reference IVF Recipes (Word)

Xenopus RNA Preparation (see also Mol Bio)

• Capped MRNA Prep (Seo) (Word)
• Total RNA from Cap (Word)

Xenopus Transgenesis

• Transgenesis Quick Reference Sheets Combined 12-2007 (Word)
• CSH Protocols REMI Transgenesis (PDF)
• Grainger Modified REMI Method Link (Word)

Xenopus Whole Mount in situ Hybridization

• Complete in situ Protocol 9-1999 (Word)
• In situ Combined Protocols 12-2007 (Word)
• X-gal Staining Protocol (Word)

Xenopus WUSM Facility Design and SOPs

• WUSM Facility Floor Plan (PDF)
• Xenopus Environmental Parameters (Word)

Xenopus Facility Manuals (Marine Biotech)

• 776C WASH FLOOR-B (PDF)
• 776C WU SYSA LABELING (PDF)
• 776C WU SYSA PFS AUTOMATION (PDF)
• 776C WU SYSB PFS AUTOMATION (PDF)
• 776C WU SYSC PFS AUTOMATION (PDF)
• Marine Biotech XR3 protocols (PDF)
• Screenviews from facility database (PDF)
• System Maintenance Schedule (PDF)
• U Wash. Floor Plan (PDF)
• U Wash. Floor Plan-C (PDF)